Antifungal effects of low level laser on Candida species with using Methylene blue and toluidine blue dayes

Introduction: Oral Candidiasis is the most common lesion in Immuno compromised patients. Using antifungal drugs are routine treatment for this condition. The aim of this study was incorporating photodynamic therapy with low level laser (685 nm), in presence of 2 dyes (methylene blue and tolidine blue), as a photosensitizer on C. albilcans, C. glabrata. C. krusie and C.tropicalis. Methods: In this experimental study, 60 samples (10 sample in 6 group) from candida suspension were radiated with laser (685 nm – 28 j/cm2) in presence or absence of methylene blue or toluidine blue. 0.1 mg/ml from each sample serial dilutions of 10-2 and 10-3 were obtained and aliquots of 0.1 m/ of each dilution were cultured on saburaual dextrose agar. After incubation at 37c for 48h, the number of colony (CFU/ml) was counted. ANOVA was used for statistical analysis. Results: Low level laser in presence of methylene blue reduceed the number of CFU/ml in 83.2% for C.albicame and toluidine blue as 88.6%. Conclusion: Our invitro study findings, show that low level laser in presence of dye (toluidine blue more effective than methylen blue) can reduce the number of candida.